By William O. Dawson (auth.), Kenneth Palmer, Yuri Gleba (eds.)
In this quantity, the authors supply a very good assessment of the way some distance the plant viral vector box has come. The self-discipline is not any longer solely within the area of academics—there is a small, yet starting to be variety of small biotechnology businesses that make the most plant viruses because the platform for advertisement innovation in crop development, commercial product production, and human and veterinary future health care.
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Extra info for Plant Viral Vectors
1 The possible evolutionary relationships of rolling circle replication (RCR) domains within the replication-associated proteins encoded by a number of ssDNA replicons. Based on a published alignment of the *80 amino acids spanning the three RCR motifs from a diverse group of ssDNA replicons (Koonin and Ilyina 1992), this unrooted dendrogram was constructed by the neighbour joining method (Saitou and Nei 1987) using 1,000 bootstrap iterations. While bootstrap support for nodes near the centre of the dendrogram is relatively poor (all nodes with less than 30% support have been collapsed), support for all the presented nanovirus, circovirus and geminivirus groupings was in excess of 50%.
2010). Curtoviruses are vectored by leafhoppers, and topocuviruses by planthoppers. Both curto and topocuviruses have a limited distribution, only a few identified members, and infect only dicots (Stanley et al. 2005). While specific geminiviruses have relatively limited host ranges, experimental host range may be considerably widened: for example, while African cassava mosaic begomovirus (ACMV) does not naturally infect tobacco (N. tabacum) or most certainly not maize, the A genome component replicates autonomously in protoplasted cells of both plants after transfection of partially dimeric DNA constructs (Paszkowski et al.
Rybicki and D. P. ) PreCP (AV2) CR CR CR AC4 Rep (AC1) OW Begomovirus A Component (ACMV DNA A) 2777 TrAP (AC2) Sat-DNA (ToLCV-Au DNA-2) 682 NW Begomovirus A Component (BGMV DNA A) 2644 Rep (AC1) CP (AV1) Begomovirus B Component (BGMV DNA B) 2609 CP (AV1) MPB (BC1) REn (AC3) REn (AC3) NSP (BV1) TrAP (AC2) β Component (AYVV-DNA β) 1347 C1 Nanovirus (MVDV-C1) 1007 Nanovirus-like DNA (AYVV-DNA1) 1367 Rep Begomovirus associated DNAs CP Circovirus (PCV) 1758 Rep Rep Replicons related to geminiviruses (b) DNA-R M-Rep 1003nt DNA-S CP 992nt DNA-N NSP 981nt DNA-U1 U1 986nt DNA-C Clink 994nt DNA-U2 U2 984 nt DNA-M MP 980nt DNA-U4 U4 987 nt invariant nonanucleotide sequence TAATATTAC in the loop forming the genomic (+) strand origin of replication.