Molecular detection of human viral pathogens by Dongyou Liu

By Dongyou Liu

Despite being famous and fought opposed to over numerous centuries, human viral pathogens proceed to reason significant public illnesses worldwide―killing thousands of individuals and costing billions of greenbacks in treatment and misplaced productiveness every year. With contributions from experts of their respective parts of viral pathogen examine, Molecular Detection of Human Viral Pathogens presents a competent reference on molecular detection and identity of significant human viral pathogens.

Each bankruptcy in short experiences the type, epidemiology, scientific good points, and prognosis of 1 similar viral pathogen or a gaggle of them. The medical pattern assortment and coaching strategies are defined, and a range of consultant stepwise molecular detection protocols is roofed. The chapters finish with a dialogue on extra study standards in terms of more advantageous analysis.

With its really apt choice of streamlined, ready-to-use protocols for significant human viral pathogens―including advertisement kits―Molecular Detection of Human Viral Pathogens is an critical software for scientific, veterinary, and commercial laboratory scientists fascinated with virus determination.

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M. et al. ), Virus Taxonomy: VIIIth Report of the International Committee on Taxonomy of Viruses (ICTV), Elsevier Academic Press, New York, 2005. 1 Classification and Property of RNA Virus Families that Are Infective to Humans Introductory Remarks 3 28–45 125–300 130–375 Double Double Double Adenoviridae Herpesviridae Poxviridae Linear (covalently closed) Linear (1) Linear (1) Circular Linear (1) Circular (1) (not covalently closed) Circular (1) Circular (1) Structure (Segment) Isometric/70–90 Spherical/150 Brick-shaped or oval/160 × 260 + + Isometric/40 Isometric/55 Isometric/17–24 Isometric/25 Spherical/30–34 — — — — — + Envelope Virion Shape/Size (nm) Complex Icosahedral Icosahedral Icosahedral Icosahedral Icosahedral Icosahedral Icosahedral Virion Nucleocapsid Symmetry Droplet contact, fecal-oral, venereal, direct contact Direct contact (blood, tears, urine, semen, saliva, vaginal secretions, lesions), mother’s milk, birth Direct contact, insect bite Direct contact (blood) Aerosol, direct contact Blood, semen, saliva, mother’s milk Aerosol, direct contact Direct contact Transmission Route Variola (small pox), vaccinia virus Human herpesviruses 1-8 JC virus, BK virus Human papilloma viruses Human adenoviruses TT Virus Bocavirus, B19 Hepatitis B virus Examples Sources: Adapted from Knipe, D.

A large number of viruses have been shown to prosper in both animals and humans, and cause zoonotic diseases. , Flaviviridae, Togaviridae, Rhabdoviridae, Bunyaviridae, and Reoviridae) that are maintained in nature involving arthropod vectors (mostly mosquitoes, sandflies, and ticks) and that are transmitted to animals including humans through insect blood feeding and bites as well as inhalation of virus-containing aerosols. 1 and also in subsequent chapters in this book, the Flaviviridae and Togaviridae families consist of positive-sense ssRNA viruses, the Rhabdoviridae family contains negative-sense ssRNA viruses, and the families Bunyaviridae and Reoviridae comprise negative sense or ambisense ssRNA or dsRNA viruses.

15. , Linkage between the journal and quality control molecular diagnostics (QCMD), J. Clin. , 27, 211, 2003. 16. , et al. Validation of assembled nucleic acid-based tests in diagnostic microbiology laboratories, Pathology, 36, 45, 2004. 17. , SARS molecular detection external quality assurance, Emerg. Infect. , 10, 2200, 2004. 18. Niesters, H. , 10, 5, 2004. 19. , and Hammerschlag, M. , In-house nucleic acid amplification assays in research: How much quality control is needed before one can rely upon the results?

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