By Josephine A Morello; Helen Eckel Mizer; Marion E Wilson
This microbiology laboratory handbook is designed in particular for the non-majors, future health technological know-how microbiology classes. The association displays the physique platforms process and includes particular sections on medical analysis. 36 workouts and forty three experiments hide a vast variety of subject matters
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Extra resources for Laboratory manual and workbook in microbiology : applications to patient care
Because any acid-fast bacilli take up this dye and fluoresce brightly against a dark background when viewed with a fluorescence microscope, the smear can be examined under 400ϫ (high-dry) magnification rather than 1,000ϫ (oil-immersion) magnification. As a result, the slide can be screened more quickly for the presence of acid-fast bacilli (see colorplate 9). Acid-Fast Stain 43 Morello−Mizer−Granato: Laboratory Manual and Workbook in Microbiology, 7/e I. Basic Techniques of Microbiology 3. Differential Stains © The McGraw−Hill Companies, 2003 Purpose To learn the acid-fast technique and to understand its value when used to stain a clinical specimen Materials A young slant culture of Mycobacterium phlei (a saprophyte) 24-hour broth culture of Bacillus subtilis A sputum specimen simulating that of a 70-year-old man from a nursing home, admitted to the hospital with chest pain and bloody sputum Gram-stain reagents Kinyoun’s carbolfuchsin Acid-alcohol solution Methylene blue Slides Diamond glass-marking pencil Marking pencil or pen 2 ϫ 3-cm filter paper strips Slide rack Forceps Procedures 1.
When an agar plate is inoculated, why is the loop sterilized after the initial inoculum is put on? 2. Define a pure culture, a mixed culture. 3. Define a bacterial colony. List four characteristics by which bacterial colonies may be distinguished. 4. Why should a petri dish not be left open for any extended period? 5. Why does the streaking method you used to inoculate your plates result in isolated colonies? Streaking Technique to Obtain Pure Cultures 63 Morello−Mizer−Granato: Laboratory Manual and Workbook in Microbiology, 7/e I.
10. 1 ml of broth culture run into the plate bottom. 11. Replace the dish top and discard the pipette into a container of disinfectant. 12. Pick up the tube of melted, cooled agar, remove its closure, and put it down on the bench top. 13. With your free hand, remove the top of the petri dish (again, do not put it down). Quickly pour the agar into the dish. 14. Replace the petri dish cover (the tube may be set aside for washing). Gently rock the closed dish, or rotate it in circular fashion on the bench top, being careful not to allow the still melted agar to wave up over the edge of the bottom half or onto the cover.