By Jerome J. Wolken
Tried to hide metabolic concerns already reviewed in Volumes I and II of The Biochemistry and body structure of Protozoa (eds. Lwoff, and Lwoff and Hutner). to these attracted to the wider elements of photograph synthesis and photoreception, reference is made to Photosynthesis and similar methods, quantity I, and elements I and a couple of of quantity II, through E. Rabinowitch; the Brookhaven Symposium at the Photochemical Ap paratus: Its constitution and serve as (1959); the hot York Academy of Sciences convention on Photoreception (1958), and to the numerous contemporary symposia, stories, and present magazine literature. This booklet is basically inquisitive about using Euglena in examine ing photoreception; it's also was hoping that biologists, biochemists, and biophysicists will locate in Euglena a remarkably flexible examine instrument for attacking a few of their difficulties. i want to thank Drs. S. H. Hutner and L. Provasoli of the Haskins Laboratories for introducing me to Euglena as a superb experimental "animal," in addition to for lots of stimulating discussions and persevered enthusiasm. i'm indebted to Drs. G. E. Palade and ok. R.
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Additional resources for Euglena: An Experimental Organism for Biochemical and Biophysical Studies
Pigments FIG. 19. 39 Molecular model of chlorophyll. / > '" I I C C CH 3 CH I g II Chlorophyll a Chlorophyll b Chlorophyll a and b differ in absorption spectra, as shown in Fig. 20, as well as in solubility; for example, chlorophyll a is more soluble in petroleum ether while chlorophyll b is more souble in methyl alcohol. Pheophytin (a or b) is chlorophyll minus magnesium. This is obtained by treatment of the chlorophyll extracts with dilute acids. " Seeds. and etiolated plants (seedlings sprouted in darkness) are sometimes faintly green, although they contain no chlorophyll.
Gracilis is a hardy laboratory weed among the euglenas. Hutner, Provasoli, Pringsheim, and others have helped shape Euglena and many protozoan forms into important tools in nutritional reesarch; their findings have cleared the way to investigations of the metabolism of Euglena. Let us consider the nutrition of Euglena. Unlike land plants, Euglena cannot utilize nitrates; it will grow rapidly if given ammonia supplemented with amino acids. NO, Light, CO" NH. Dark, butyrate, NH. Light, CO, NH. Dark, butyrate, NH.
27. Absorption spectrum of young Euglena culture grown at low light level. 2 fL. 6 X 10- 14 liters. This number of chlorophyll molecules per chloroplast is in agreement with previous determinations for Euglena and Chiarella chloroplasts (Wolken 52 Euglena and Schwertz, 1953; Wolken, 1956a; Olson and Engle, 1959; Strother and Wolken, 1961). Eyespot The bright orange-red color of the eyespot indicates the presence of a concentrated pigment. An orange-red fraction was isolated by differential centrifugation after grinding the cells.