African Horse Sickness by Philip S. Mellor, Matthew Baylis, Christopher Hamblin,

By Philip S. Mellor, Matthew Baylis, Christopher Hamblin, Charles H. Calisher, Peter P.C. Mertens

African horse illness virus is a double-stranded RNA virus which factors a non-contagious, infectious arthropod-borne ailment of equines and infrequently canine. 9 specific, the world over acknowledged serotypes of the virus have thus far been pointed out. This ebook relies upon the findings of 2 programmes funded by means of the ecu fee. it will likely be of worth not just to the expert examine employees but additionally to veterinary staff facing regulate and to legislators looking to advertise secure overseas move of equines. the subjects coated contain cutting-edge discussions on diagnostics, vaccines, molecular biology, vector stories, and epidemiology.

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The resultant pellet was resuspended in 15 ml of PBS and the suspension layered onto 5 microscope slides. The slides were dried, fixed in cold acetone (-20°C) for 30 min and then stored at -70°C until required. Donkey hyperimmune AHSV antiserum was layered onto the fixed cell suspensions and the slides were incubated at 37°C for 45 min. Slides were then washed in PBS, blotted dry and previously titrated, fluoescein isothiocyanate conjugated rabbit anti-donkey IgG (diluted to 1: 80 in PBS) added, before incubation at 37°C for a further 30 min.

Theiler A (1921) African horse sickness (Pestis equorum). 5th Afr Dept Ag Sci Bull No 19 16. Theiler A (1930) African horse sickness. Medical Research Council. A system of bacteriology in relation to medicine, vol 7, chapter 31: Virus diseases - Bacteriophages. HSMO, London, pp 362-375 17. Venter GJ, Williams R, Nevill EM (1992) The value of donkeys as sentinels for African horse sickness. In: Walton TE, Osburn BI (eds) Bluetongue, African horse sickness and related orbiviruses. Proc 2nd Int Symp Paris 1991.

Viruses The live, attenuated AHSV 4 monovalent vaccine, Lot No R117-4/94 was prepared and supplied by Biopharma, Rabat, Morocco. The wild-type AHSV was isolated in embryonated hens eggs following intravenous inoculation of 20 Ili/egg of homogenised spleen tissue, diluted 1 in 10 in phosphate buffered saline (PBS), that had been taken from a horse that died of AHS in Morocco. 9 ELDsofm1. Study A The 4 donkeys and 4 mules were each inoculated by the sub-cutaneous route into the side of the neck with a single dose of vaccine according to the manufacturer's instructions.

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